![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
Part:BBa_K4458014:Design
Designed by: Maya Balakumaran Group: iGEM22_East_Coast_BioCrew (2022-10-01)
Cytochrome P450 Family 1 Subfamily A Member 1(CYP1A1)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1426
Illegal XbaI site found at 3411
Illegal PstI site found at 944
Illegal PstI site found at 1021 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2620
Illegal PstI site found at 944
Illegal PstI site found at 1021 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 670
Illegal BglII site found at 721
Illegal BglII site found at 1111
Illegal BamHI site found at 697 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1426
Illegal XbaI site found at 3411
Illegal PstI site found at 944
Illegal PstI site found at 1021 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1426
Illegal XbaI site found at 3411
Illegal PstI site found at 944
Illegal PstI site found at 1021
Illegal NgoMIV site found at 2634 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1844
Illegal BsaI.rc site found at 266
Design Notes
Due to high and low GC content, we had to modify a specific part of the sequence. We used Twist's codon optimization tool to reduce GC content. We tried to conserve regulatory regions in the fragment.
Source
NG_061374 Homo sapiens cytochrome P450 family 1 subfamily A member 1(CYP1A1), RefSeqGene (LRG_1262) on chromosome 15.